Extended-spectrum β-lactamase-producing enterobacteriaceae causing nosocomial infections in Mexico. A retrospective and multicenter study.
作者： Jesus Silva-Sanchez , Jesús Ulises Garza-Ramos , Fernando Reyna-Flores , Alejandro Sánchez-Pérez , Teresa Rojas-Moreno
DOI: 10.1016/J.ARCMED.2011.02.004
关键词: Agar diffusion test 、 Enterobacteriaceae 、 Antibiotics 、 Microbiology 、 Bacteria 、 Cephalosporin 、 Enterobacter cloacae 、 Agar dilution 、 Biology 、 Klebsiella pneumoniae
摘要: Abstract Background and Aims Extended-spectrum β-lactamase (ESBLs) production is still the most frequent mechanism of resistance to cephalosporins in gram-negative bacteria. The aim study was identify types ESBL-producing Enterobacteriaceae clinical isolates causing nosocomial infections Mexico. Methods ESBL performed using a disk diffusion method. MIC for several antibiotics by agar dilution on Mueller-Hinton. PFGE typing carried out all enterobacteria assayed. pattern obtained IEF bioassay. Genes β-lactamases were amplified PCR with specific primers products sequenced analyzed informatics programs. Plasmid isolation conjugation experiments standard methodologies. Results There 134 included from retrospective multicenter that eight Mexican hospitals 1999 2005. prevalent species K. pneumoniae (56%), Enterobacter cloacae (29%), Escherichia coli (15%). Molecular analysis identified underlying endemic polyclonal spread enterobacterials each hospital. ESBLs SHV-type (84%), TLA-1 (11%), CTX-M-15 (5%). Successful matings detected 68.4% (71/104) isolates. Conclusions ESBL-producer remains bacterial infections. disseminated emerging one studied hospitals. This work highlights proper use avoid selection these multiresistant
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