Fibroblast growth factor promotes survival of dissociated hippocampal neurons and enhances neurite extension.

摘要: Abstract Basic fibroblast growth factor (FGF) has been found to increase neuronal survival and neurite extension in a highly purified population of fetal rat hippocampal neurons under well-defined serum-free cell culture conditions. In the presence FGF, after 7 days on simple plastic substrate is increased 4-fold, 54% initial population. Survival 2-fold 40% polyornithine-laminin. When FGF was bound or heparin substrates, outgrowth significantly lengths comparable those seen with laminin; however, produced no further laminin. Half-maximal observed at concentrations about 15 pg/ml (1 pM); half-maximal process occurred 375 (20 pM). The responsive cells were identified as by their labeling tetanus toxin antibodies neurofilaments neuron-specific enolase. Astrocytes, glial fibrillary acidic protein, constituted 10% present 1 week both absence FGF. These results strongly suggest that, addition its known mitogenic effects nonneuronal cells, possesses neurotrophic activity for neurons.