Production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with Kluyveromyces marxianus
作者： Chao-Chun Cheng , Mei-Ching Yu , Tzu-Chien Cheng , Dey-Chyi Sheu , Kow-Jen Duan
DOI: 10.1007/S10529-006-9002-1
关键词: Kluyveromyces 、 Fermentation 、 Kluyveromyces marxianus 、 Oligosaccharide 、 Kluyveromyces lactis 、 Aspergillus oryzae 、 Galactose 、 Chemistry 、 Lactose 、 Food science 、 Biochemistry
摘要: Of three β-galactosidases from Aspergillus oryzae, Kluyveromyces lactis and Bacillus sp., used for the production of low-content galacto- oligosaccharides (GOS) lactose, latter produced highest yield trisaccharides tetrasaccharides. GOS was enhanced by mixing β-galactosidase glucose oxidase. The syrups, either alone or mixed enzyme system, were subjected to fermentation marxianus, whereby glucose, galactose, lactose other disaccharides depleted, resulting in up 97% 98% on a dry weight basis high-content with yields 31% 32%, respectively.
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