Assessment of Dog Testis Perfusion by Colour and Pulsed-Doppler Ultrasonography and Correlation With Sperm Oxidative DNA Damage.
作者： Helena Lemos , Jesus Dorado , Manuel Hidalgo , Isabel Gaivão , Ana Martins-Bessa
DOI: 10.1016/J.TCAM.2020.100452
关键词: Testicular artery 、 DNA damage 、 Perfusion 、 Left Testis 、 Comet assay 、 Blood flow 、 Oxidative dna damage 、 Sperm 、 Medicine 、 Andrology
摘要: Abstract The assessment of testicular artery blood flow by colour and pulsed-Doppler ultrasonography is an important diagnostic technique to assess vascular perfusion. Recently, it has been suggested as a good predictor sperm quality. On the other hand, through alkaline Comet Assay, possible quantify oxidative DNA damage. aim this study was evaluate relationship between routine parameters, damage in canine sperm. Testicular collection were performed on 12 male dogs, with animals being allocated into 2 groups, according classification ejaculates’ quality, normozoospermic (N; n = 7) or non-normozoospermic (OAT; n = 5). Seven dogs aged 1.5 8.0 years old included group N 5 2.0 11.0 old, OAT. sperm-rich fraction ejaculates evaluated for parameters comet assay. Colour used supratesticular marginal arteries right left testis. Group OAT presented higher levels (A.U.) comparison (N:11.7 ± 9.9; OAT:34.2 6.1; P
sci-hub.st 参考文章(27)
Mírley Barbosa de Souza, Gary C.W. England, Antônio Cavalcante Mota Filho, Camila Louise Ackermann, Carmen Vládia Soares Sousa, Gabriela Guedelha de Carvalho, Herlon Victor Rodrigues Silva, José Nicodemos Pinto, Jussiara Candeira Spíndola Linhares, Eunice Oba, Lúcia Daniel Machado da Silva, Semen quality, testicular B-mode and Doppler ultrasound, and serum testosterone concentrations in dogs with established infertility Theriogenology. ,vol. 84, pp. 805- 810 ,(2015) , 10.1016/J.THERIOGENOLOGY.2015.05.015
JD Carrillo, M Soler, X Lucas, A Agut, None, Colour and Pulsed Doppler Ultrasonographic Study of the Canine Testis Reproduction in Domestic Animals. ,vol. 47, pp. 655- 659 ,(2012) , 10.1111/J.1439-0531.2011.01937.X
Jennifer L. Giffin, Sarah E. Franks, Jose Rafael Rodriguez-Sosa, Ann Hahnel, Pawel M. Bartlewski, A Study of Morphological and Haemodynamic Determinants of Testicular Echotexture Characteristics in the Ram Experimental Biology and Medicine. ,vol. 234, pp. 794- 801 ,(2009) , 10.3181/0812-RM-364
Pagona G. Gouletsou, Apostolos D. Galatos, Leonidas S. Leontides, Comparison between ultrasonographic and caliper measurements of testicular volume in the dog Animal Reproduction Science. ,vol. 108, pp. 1- 12 ,(2008) , 10.1016/J.ANIREPROSCI.2007.06.020
Juan G. Reyes, Jorge G. Farias, Sebastián Henríquez-Olavarrieta, Eva Madrid, Mario Parraga, Andrea B. Zepeda, Ricardo D. Moreno, The hypoxic testicle: physiology and pathophysiology. Oxidative Medicine and Cellular Longevity. ,vol. 2012, pp. 929285- 929285 ,(2012) , 10.1155/2012/929285
C Ortega‐Ferrusola, LA Gracia‐Calvo, J Ezquerra, FJ Pena, None, Use of colour and spectral Doppler ultrasonography in stallion andrology Reproduction in Domestic Animals. ,vol. 49, pp. 88- 96 ,(2014) , 10.1111/RDA.12363
Mírley Barbosa de Souza, Claudia da Cunha Barbosa, Barbara Sucupira Pereira, Cynthia Levi Baratta Monteiro, José Nicodemos Pinto, Jussiara Candeira Spíndola Linhares, Lúcia Daniel Machado da Silva, Doppler velocimetric parameters of the testicular artery in healthy dogs Research in Veterinary Science. ,vol. 96, pp. 533- 536 ,(2014) , 10.1016/J.RVSC.2014.03.008
Clara González-Marín, Jaime Gosálvez, Rosa Roy, Types, Causes, Detection and Repair of DNA Fragmentation in Animal and Human Sperm Cells International Journal of Molecular Sciences. ,vol. 13, pp. 14026- 14052 ,(2012) , 10.3390/IJMS131114026
Harriet J. Paltiel, David A. Diamond, James Di Canzio, David Zurakowski, Joseph G. Borer, Anthony Atala, Testicular volume: comparison of orchidometer and US measurements in dogs. Radiology. ,vol. 222, pp. 114- 119 ,(2002) , 10.1148/RADIOL.2221001385
Malgorzata A. Pozor, Evaluation of Testicular Vasculature in Stallions Clinical Techniques in Equine Practice. ,vol. 6, pp. 271- 277 ,(2007) , 10.1053/J.CTEP.2007.09.007