The RecQ helicase Sgs1 drives ATP-dependent disruption of Rad51 filaments.
作者： J Brooks Crickard , Chaoyou Xue , Weibin Wang , Youngho Kwon , Patrick Sung
DOI: 10.1093/NAR/GKZ186
关键词: Sgs1 、 Cell biology 、 RecQ helicase 、 Replication protein A 、 Biology 、 Bloom syndrome 、 Helicase 、 Recombinase 、 Mutation 、 RAD51
摘要: DNA helicases of the RecQ family are conserved among three domains life and play essential roles in genome maintenance. Mutations several human lead to diseases that marked by cancer predisposition. The Saccharomyces cerevisiae helicase Sgs1 is orthologous BLM, defects which cause cancer-prone Bloom's Syndrome. Here, we use single-molecule imaging provide a quantitative mechanistic understanding activities on single stranded (ssDNA), central intermediate all aspects metabolism. We show acts upon ssDNA bound either replication protein A (RPA) or recombinase Rad51. Surprisingly, find utilizes novel motor mechanism for disrupting intermediates ability disrupt Rad51-ssDNA filaments may explain some engendered RECQ deficiencies cells.
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