Redox regulation of microRNAs in endometriosis-associated pain.

摘要: Endometriosis is a chronic, painful condition with unknown etiology. A differential expression of microRNAs in the endometriotic tissues from women endometriosis pain compared to those without suggested plausible role for miRNA or epigenetic mechanisms etiology pain. The peritoneal milieu involved maintenance lesion and nociception. We recently showed mechanistic oxidized-lipoproteins (ox-LDLs) present fluid (PF) explored possibility ox-LDLs modulating miRNAs manner similar PF endometriosis. Expression levels their predicted nociceptive inflammatory targets were determined ox-LDL treated human endometrial cell-lines. Samples IRB-approved consented patients used. These cell-lines various forms oxidized-lipoproteins. RNA (including miRNAs) isolated cells using commercial miRNome arrays. Cell lysates used immunoblotting proteins protein array. Twenty including isoforms miR-29, miR-181 let-7 mutually differentially expressed components. endo-PF treatment also produced significant overexpression microRNA target genes nerve growth factor, interleukin-6 prostaglandin E synthase downstream Mip1α MCP1. This study similarities between regulation abundance these women. Key responsible targeting molecules downregulated presence endo-PF, thus playing redox-sensitive can be potential use as endometriosis-associated