Isolation of Plesiomonas shigelloides from Infected Cichlid Fishes using 16S rRNA Characterization and its Control with Probiotic Pseudomonas sp.

摘要: Background: Bacterial diseases are reported to cause heavy mortalities in both cultured and wild fi shes throughout the world. In any eld, diagnosis of disease plays a main role curing disease. aquaculture, antibiotics used control infectious caused by bacteria. The use has been questioned as it experiences problems bacterial resistance antimicrobials unacceptable residues aquaculture products environment. Recently, many studied have performed application probiotic measure diseases. Therefore, this study designed for isolation possible bacteria which inhibit growth specifi c pathogenic isolated from fresh water ornamental sh. Materials, Methods & Results: Diseased cichlid were brought laboratory causative A total three strains (APG01, APG02 FA3) serially diluted samples gut, gill spleen. injected at concentration 10 5 cfu/mL healthy fiout its virulency vivo varying time duration (12, 24, 36 48 h) exposure 15 days. Among isolates, only APG01 100% mortality h exposure. was subjected 16S rRNA characterization confi rmed Plesiomonas shigelloides. nucleotide sequence submitted NCBI Banklt provided with accession number. To vitro inhibition shigelloides, (Pseudomonas strain BPH2) fermented rice. antibacterial activity agar well diffusion assay 10, 50 100 µL 7 cfu/mL. Maximum zone 23 ± 0.78 mm produced Pseudomonas BPH2. Also, effect cell free supernatant observed 21 1.00 against P. shigelliodes. susceptibility reproducibility pH tested observing media ranging 9. measured absorbency 600 nm every 8 h. BPH2 found susceptible 6 Discussion: assay, rice showed maximum inhibitory shigelloides APG01. report Lactococcus lactis milk 14.77 1.17 Aeromonas hydrophila assay. While comparing result, is maximum. tolerance revealed that could signifi cantly infl uence viability Lactobacillus mesenteroides survival 3 - highest rate neutral conditions. between 8. Hence failed show acidic pH, might be questionable gut associated probiotic. Other than can survive aquatic environment effectively system like soil