Determination of linear fluorescence intensities from flow cytometric data accumulated with logarithmic amplifiers.
作者： K. A. Muirhead , T. C. Schmitt , A. R. Muirhead
关键词: Calibration curve 、 Amplifier 、 Logarithm 、 Fluorescence 、 SPHERES 、 Materials science 、 Biological system 、 Stability (probability) 、 Linear space 、 Intensity (heat transfer)
摘要: Logarithmic amplifiers are useful in accumulating flow cytometric data with a large dynamic range. However, quantitative comparison of fluorescence intensities for different samples or subpopulations within sample is simplified by the conversion from log space back to linear space. A method described which fluorescent polystyrene spheres differing used construct calibration curve logarithmic intensity scale. This allows calculation relative each channel logarithmically accumulated and determination means coefficients variation comparative purposes. Fluorescent appropriate may also be as internal standards monitor instrument and/or stain stability using amplifiers.
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