Formation of blood-group A substance from H substance by an alpha-N-acetylgalactosaminyl transferase. Partial purification of the enzyme from hog gastric mucosa.
作者： H. Tuppy , H. Schenkel-Brunner
DOI: 10.1111/J.1432-1033.1969.TB00667.X
关键词: Hemagglutination Inhibition Tests 、 Chromatography 、 Helix pomatia 、 Acetylgalactosamine 、 Biochemistry 、 Enzyme 、 Transferase 、 Chemistry 、 Ion chromatography 、 Microsome 、 Glycosyl donor
摘要: An α-N-acetylgalactosaminyl transferase has been solubilized from hog gastric mucosal microsomes and purified approximately 14-fold by ion exchange chromatography. The enzyme catalyzes the transfer of tritum-labeled N-acetyl-d-galactosamine UDP-[3H]acetylgalactosamine to blood-group H substance. The net synthesis A substance was demonstrated using hemagglutination inhibition tests. newly fromed activity shown be susceptible destruction N-acetylgalactosaminidase Helix pomatia. Part enzymatically synthesized radioactive could precipitated with human anti-A serum. The a sharp pH optimum at 6.9 requires Mn++ for maximum activity. Km value glycosyl donor, UDP-acetylgalactosamine, is 33 μM.
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