Low-temperature femtosecond spectroscopy of the initial step of electron transfer in reaction centers from photosynthetic purple bacteria
作者： J. Breton , J. L. Martin , G. R. Fleming , J. C. Lambry
DOI: 10.1021/BI00421A043
关键词: Photosynthetic bacteria 、 Electron transfer 、 Rhodobacter sphaeroides 、 Chemistry 、 Bacteriochlorophyll 、 Absorption spectroscopy 、 Photosynthetic reaction centre 、 Purple bacteria 、 Analytical chemistry 、 Photochemistry 、 Absorption band
摘要: The initial step of charge separation at 10 K has been monitored with 100-fs time resolution in reaction centers from Rhodopseudomonas viridis and Rhodobacter sphaeroides as well the latter species which one two monomeric bacteriochlorophyll (B) molecules removed by treatment borohydride. Upon excitation 870 nm, absorbance changes measured several wavelengths near-infrared absorption bands pigments, notably maximum B molecule(s), give no indication a detectable concentration B-. Instead, appearance cation radical dimeric primary electron donor (P) bacteriopheophytin anion develops concert decay P*. An bleaching 850-nm band Rho- dopseudomonas uiridis is consistent an assignment least large fraction this to high-energy exciton component P. molecule(s) around 600 nm three types investigated, ultrafast energy transfer leads formation P* less than 100 fs. Under these conditions, fast transient decaying 400-fs constant observed within B. This also present upon preferential bac- teriopheophytins center uiridis.
acs.org
osti.gov
berkeley.edu 参考文章(49)
V.A. Shuvalov, A.V. Klevanik, The study of the state [P870+
B800−
] in bacterial reaction centers by selective picosecond and low-temperature spectroscopies FEBS Letters. ,vol. 160, pp. 51- 55 ,(1983) , 10.1016/0014-5793(83)80934-X
Sighart F. Fischer, P.O.J. Scherer, On the early charge separation and recombination processes in bacterial reaction centers Chemical Physics. ,vol. 115, pp. 151- 158 ,(1987) , 10.1016/0301-0104(87)80029-0
Dan W. Reed, Roderick K. Clayton, Isolation of a reaction center fraction from Rhodopseudomonas spheroides Biochemical and Biophysical Research Communications. ,vol. 30, pp. 471- 475 ,(1968) , 10.1016/0006-291X(68)90075-2
J. W. Petrich, J. L. Martin, D. Houde, C. Poyart, A. Orszag, Time-resolved Raman spectroscopy with subpicosecond resolution: vibrational cooling and delocalization of strain energy in photodissociated (carbonmonoxy)hemoglobin. Biochemistry. ,vol. 26, pp. 7914- 7923 ,(1987) , 10.1021/BI00398A056
Vladimir A. Shuvalov, William W. Parson, Triplet states of monomeric bacteriochlorophyll in vitro and of bacteriochlorophyll dimers in antenna and reaction center complexes Biochimica et Biophysica Acta. ,vol. 638, pp. 50- 59 ,(1981) , 10.1016/0005-2728(81)90184-5
M.E. Michel-Beyerle, M. Plato, J. Deisenhofer, H. Michel, M. Bixon, J. Jortner, Unidirectionality of charge separation in reaction centers of photosynthetic bacteria Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 932, pp. 52- 70 ,(1988) , 10.1016/0005-2728(88)90139-9
G. Paillotin, A. Vermeglio, J. Breton, Orientation of reaction center and antenna chromophores in the photosynthetic membrane of rhodopseudomonas viridis Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 545, pp. 249- 264 ,(1979) , 10.1016/0005-2728(79)90204-4
S.F. Fischer, E.W. Knapp, P.O.J. Scherer, Model studies of low-temperature optical transitions of photosynthetic reaction centers A-, LD-, CD-, ADMR- and LD-ADMR-spectra for Rhodopseudomonas viridis Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 852, pp. 295- 305 ,(1986) , 10.1016/0005-2728(86)90235-5
H.J. Den Blanken, A.P.J.M. Jongenelis, A.J. Hoff, The triplet state of the primary donor of the photosynthetic bacterium Rhodopseudomonas viridis Biochimica et Biophysica Acta (BBA) - Bioenergetics. ,vol. 725, pp. 472- 482 ,(1983) , 10.1016/0005-2728(83)90188-3
S. Creighton, J. K. Hwang, A. Warshel, W. W. Parson, J. Norris, Simulating the dynamics of the primary charge separation process in bacterial photosynthesis Biochemistry. ,vol. 27, pp. 774- 781 ,(1988) , 10.1021/BI00402A043