Annotation of the Staphylococcus aureus Metabolome Using Liquid Chromatography Coupled to High-Resolution Mass Spectrometry and Application to the Study of Methicillin Resistance.

摘要: Staphylococcus aureus can cause a variety of severe disease patterns and readily acquire antibiotic resistance; however, the mechanisms by which this commensal becomes pathogen or develops resistance are still poorly understood. Here we asked whether metabolomics be used to distinguish bacterial strains with different susceptibilities. Thus, an efficient robust method was first thoroughly implemented measure intracellular metabolites S. in unbiased reproducible manner. We also placed special emphasis on metabolome coverage annotation both hydrophilic interaction liquid chromatography pentafluorophenyl-propyl columns coupled high-resolution mass spectrometry conjunction our spectral database developed in-house identify high confidence as many meaningful possible. Overall, were able characterize up 210 aureus, represents substantial ∼50% improvement over previously published data. then preliminarily compared metabolic profiles 10 clinically relevant methicillin-resistant susceptible harvested at time points during exponential growth phase (without any exposure). Interestingly, resulting data revealed distinct behavior "slow-growing" resistant strains, show modified levels several precursors peptidoglycan capsular polysaccharide biosynthesis.