Cloning, expression and characterization of mugwort pollen allergen Art v 2, a pathogenesis-related protein from family group 1
作者： M.C. Arilla , I. Ibarrola , Y. Puente , J.C. Daza , A. Martínez
DOI: 10.1016/J.MOLIMM.2007.04.022
关键词: Immunology 、 Allergen 、 Cloning 、 Molecular biology 、 Complementary DNA 、 Mugwort 、 Biology 、 Homology (biology) 、 Molecular mass 、 Recombinant DNA 、 Artemisia vulgaris
摘要: Mugwort (Artemisia vulgaris) belongs to the Compositae family, and is one of main causes allergy in late summer autumn. The aim study was characterize allergen Art v 2 from mugwort pollen. Skin prick tests, performed 19 patients allergic 10 control patients, showed an sensitization prevalence 58%, whereas none false-positives were detected among patients. purified by standard chromatography binding Concanavalin A column had apparent molecular mass 33 20 kDa, calculated gel permeation SDS-PAGE under denaturing conditions, respectively, showing that composed two identical subunits. 2-encoding cDNA amplified PCR using degenerate primers based on reported partial amino acid sequences. Cloned encoding contains 140 bp codify for a polypeptide 15.8 with predicted pI value 5.2, potential N-glycosylation site. Protein homology search demonstrated share 55-42% residues pathogenesis-related protein PR-1 tomato, potato, rape, wheat rice. Homology also found Ves 5 (41% residues). Bacterial-expressed recombinant recognized only 21% mugwort-allergic In conclusion, first weed pollen its form could help diagnosis associated allergy.
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sci-hub.se 参考文章(32)
Julie D. Thompson, Desmond G. Higgins, Toby J. Gibson, CLUSTAL W: IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE Nucleic Acids Research. ,vol. 22, pp. 4673- 4680 ,(1994) , 10.1093/NAR/22.22.4673
Anette Henriksen, Te P. King, Osman Mirza, Rafa�l I. Monsalve, K�re Meno, Henrik Ipsen, J�rgen N. Larsen, Michael Gajhede, Michael D. Spangfort, Major Venom Allergen of Yellow Jackets, Ves V 5: Structural Characterization of a Pathogenesis-Related Protein Superfamily. Proteins. ,vol. 45, pp. 438- 448 ,(2001) , 10.1002/PROT.1160
Martin Himly, Beatrice Jahn‐Schmid, Azra Dedic, Peter Kelemen, Nicole Wopfner, Friedrich Altmann, Ronald Ree, Peter Briza, Klaus Richter, Christof Ebner, Fátima Ferreira, Art v 1, the major allergen of mugwort pollen, is a modular glycoprotein with a defensin-like and a hydroxyproline-rich domain The FASEB Journal. ,vol. 17, pp. 106- 108 ,(2003) , 10.1096/FJ.02-0472FJE
A. DÍaz-Perales, M. Lombardero, R. SÁnchez-Monge, F. J. GarcÍa-Selles, M. Pernas, M. FernÁndez-Rivas, D. Barber, G. Salcedo, Lipid-transfer proteins as potential plant panallergens: cross-reactivity among proteins of Artemisia pollen, Castanea nut and Rosaceae fruits, with different IgE-binding capacities. Clinical & Experimental Allergy. ,vol. 30, pp. 1403- 1410 ,(2000) , 10.1046/J.1365-2222.2000.00909.X
Zheng Zhang, Webb Miller, David J Lipman, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research. ,vol. 25, pp. 3389- 3402 ,(1997) , 10.1093/NAR/25.17.3389
C RADAUER, H BREITENEDER, Pollen allergens are restricted to few protein families and show distinct patterns of species distribution. The Journal of Allergy and Clinical Immunology. ,vol. 117, pp. 141- 147 ,(2006) , 10.1016/J.JACI.2005.09.010
L.C. VAN LOON, E.A. VAN STRIEN, The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins Physiological and Molecular Plant Pathology. ,vol. 55, pp. 85- 97 ,(1999) , 10.1006/PMPP.1999.0213
U. K. Laemmli, Cleavage of structural proteins during the assemble of the head of bacterio-phage T4 Nature. ,vol. 277, pp. 680- 685 ,(1970)
M. Way, B. Pope, J. Gooch, M. Hawkins, A. G. Weeds, Identification of a region in segment 1 of gelsolin critical for actin binding. The EMBO Journal. ,vol. 9, pp. 4103- 4109 ,(1990) , 10.1002/J.1460-2075.1990.TB07632.X
B M Nilsen, K Sletten, B S Paulsen, M O'Neill, H van Halbeek, Structural analysis of the glycoprotein allergen Art v II from the pollen of mugwort (Artemisia vulgaris L.). Journal of Biological Chemistry. ,vol. 266, pp. 2660- 2668 ,(1991) , 10.1016/S0021-9258(18)52295-X