Studies on Immobilized Lipase in Hydrophobic Sol-Gel
作者： Cleide M. F. Soares , Onelia A. dos Santos , Heizir F. de Castro , Flavio F. de Moraes , Gisella M. Zanin
DOI: 10.1007/978-1-59259-837-3_26
关键词: Candida rugosa 、 Specific surface area 、 Lipase 、 Triacylglycerol lipase 、 Adsorption 、 Chemistry 、 Polyethylene glycol 、 Hydrolysis 、 Nuclear chemistry 、 Thermogravimetric analysis 、 Chromatography
摘要: The hydrolysis of tetraethoxysilane using the sol-gel process was used to produce silica matrices, and these were tested for immobilization lipase from Candida rugosa by three methods: physical adsorption, covalent binding, gel entrapment in presence absence polyethylene glycol (PEG-1450). matrices their derivatives characterized regarding particle size distribution, specific surface area, pore distribution (Brunauer, Emmett, Teller [B.E.T.] method), yield grafting (thermogravimetric analyzer [TGA]), chemical composition (Fourier transform infrared). Immobilization yields based on recovered activity varied 3.0 32.0%, highest efficiency attained when encapsulated PEG.
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