Induction and partial characterization of California halibut (Paralichthys californicus) vitellogenin.

作者: A.J. Palumbo , J. Linares-Casenave , W. Jewell , S.I. Doroshov , R.S. Tjeerdema

DOI: 10.1016/J.CBPA.2006.10.023

关键词: Mass spectrometryVitellogeninWestern blotSize-exclusion chromatographyCalifornia halibutBiologyMolecular massParalichthysMolecular biologyPolyacrylamide gel electrophoresis

摘要: The egg yolk precursor protein, vitellogenin (Vg), was isolated by size exclusion and ion exchange chromatography from plasma of California halibut (Paralichthys californicus) treated with estrogen. MALDI TOF mass spectrometry (MS) analysis resulted in a molecular 188 kDa. MS/MS de novo sequencing identified the protein as Vg matching sequences tryptic peptides to known several other species. Matches were also made two different forms haddock, medaka, mummichog, providing evidence that has more than one form Vg. Native PAGE Western blot an antibody turbot (Scophthalmus maximus) confirmed identity protein. Protein resolved on SDS double band approximately same determined TOF, lower bands immunoreactive. useful for determining mass, identification, exploring multiplicity potential using MS methods understand structure function is discussed.

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