Herpesvirus genetics has come of age

摘要: The genetic analysis of the large and complex herpesviruses has been a constant challenge to herpesvirologists. Elegant methods have developed produce mutants in infected cells that rely on cellular recombination machinery. Bacterial artificial chromosomes (BACs), single copy F-factor-based plasmid vectors intermediate insert capacity, now enabled cloning complete herpesvirus genomes. Infectious virus genomes can be shuttled between Escherichia coli eukaryotic cells. Herpesvirus BAC DNA engineering E. by homologous requires neither restriction sites nor steps allows introduction wide variety modifications. Such coli-based technology provided safe, fast effective approach systematic mining information stored as result their intimate co-evolution with specific hosts for millions years. Use this technique could lead new developments clinical virology basic research, increase usage viral investigative tools vectors.