Properties of two insect cell lines useful for the baculovirus expression system in serum-free culture.
作者： Takeyuki Sugiura , Egon Amann
DOI: 10.1002/(SICI)1097-0290(19960820)51:4<494::AID-BIT13>3.0.CO;2-I
关键词: Gene expression 、 Recombinant DNA 、 Biochemistry 、 Sucrose 、 Metabolism 、 Recombinant virus 、 Baculoviridae 、 Cell culture 、 Sf9 、 Virology 、 Biology
摘要: The properties of Sf9 and Tn5 insect cells were analyzed comparatively under serum-free culture conditions. in SF900II medium apparently utilized sucrose as a primary nutrient both before after virus infection, yielding small amounts lactate ammonia. Excell 401 consumed all the nutrients examined, including sucrose. productivity recombinant glycoprotein, OSF-2, by cells, was moderate monolayer spinner cultures, but ability to secrete it compromised former case. Relative produced 30-fold more OSF-2 either mode. (c) 1996 John Wiley & Sons, Inc.
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