Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products.

摘要: Abstract Hybridomas secreting monoclonal antibodies specific for the adenovirus early region 1A (E1A) proteins were prepared from BALB/c mice immunized with a bacterial trpE-E1A fusion protein. This protein is encoded by hybrid gene that joins portion of Escherichia coli trpE and cDNA copy E1A 13S mRNA (Spindler et al., J. Virol. 49:132-141, 1984). Eighty-three hybridomas secrete which recognize immunogen isolated single cell cloned. Twenty-nine these are Only 12 can efficiently immunoprecipitate polypeptides detergent lysates infected cells. analyzed on one-dimensional, sodium dodecyl sulfate-polyacrylamide gels two-dimensional, isoelectric focusing polyacrylamide gels. The specifically immunoprecipitated heterogeneous in size charge be resolved into approximately 60 polypeptide species. heterogeneity due not only to synthesis multiple mRNAs, but also at least part post-translational modification. Several divide immunological subclasses based ability bind antigen. In particular, two synthesized mRNA, other proteins.