Evaluation of the presence of a thapsigargin-sensitive calcium store in trypanosomatids using Trypanosoma evansi as a model.
作者： M. Mendoza , A. Mijares , H. Rojas , C. Colina , V. Cervino
DOI: 10.1645/GE-263R
关键词: Endoplasmic reticulum 、 Thapsigargin 、 Biochemistry 、 Population 、 Homeostasis 、 SERCA 、 Kinetoplastida 、 ATPase 、 Biology 、 Trypanosoma evansi
摘要: Ca2+ plays an important role in the regulation of several activities different trypanosomatids. These parasites possess a transport system endoplasmic reticulum (ER) involved homeostasis, which has been reported to be insensitive thapsigargin, classical inhibitor sarcoplasmic–ER adenosine triphosphatase (ATPase) (SERCA) most eukaryotic cells. However, currently there is controversy regarding existence thapsigargin-sensitive ER store these parasites. Therefore, we decided explore effect this using methodological approaches. First, selected Trypanosoma evansi as parasite model warrant homogeneity population because only single life cycle, i.e., bloodstream-form trypomastigotes. Second, compared thapsigargin on homeostasis by spectrophotometrical measurements 3 approaches: whole-cell populations, cells that have permeabilized...
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