Effect of recombinant monokines, lymphokines, and other agents on clonal proliferation of human lung cancer cell lines

摘要: The modulation of clonal growth cells 15 human lung cancer lines was examined by coculture with different recombinant lymphokines, monokines, and several agents which induce differentiation in other malignant cell systems. Recombinant tumor necrosis factor alpha (TNF) inhibitory to all non-small a 50% effective dose inhibition (ED50) the range 30-2000 units/ml. Two representative squamous (SK-MES P3) had 150 250 high affinity (Kd approximately equal pM) surface TNF receptors. In contrast, small not inhibited TNF, two (H69c R592) expressed negligible alpha, beta, gamma interferons (4000 units/ml) each greater than or 30% more lines. (100-1000 combination gamma-interferon synergistic these cells. Further studies showed that synergism occurred even when were initially exposed gamma-interferon, washed, plated soft agar TNF. All-trans-retinoic acid (ED50, 5 X 10(-7)-10(-6) M), dimethyl sulfoxide 1.2-1.6%), 12-O-tetradecanoylphorbol-13-acetate 10(-8)-10(-10) M) proliferation 7 9, 8 9 lines, respectively. decreased only slightly at almost concentrations agents. Interleukin-1 -2 granulocyte-monocyte colony-stimulating no effect on any Our results suggest may be therapeutically active for some patients cancer, but probably will unresponsive we examined.