Effect of Metals on the Lytic Cycle of the Coccolithovirus, EhV86.

摘要: In this study we show that metals, and in particular copper (Cu), can disrupt the lytic cycle Emiliania huxleyi - EhV86 host-virus system. Numbers of virus particles produced per E. cell lysis rates were reduced by Cu at total metal concentrations over 500 nM presence EDTA (ethylenediaminetetraacetic acid), 250 absence acute short term exposure experiments. Zinc (Zn), cadmium (Cd) cobalt (Co) not observed to affect rate these The cellular glutathione (GSH) content increased infected cells, but as a result exposure. contrast, phytochelatins (PCs) only response increase gluthatione is consistent with increases production reactive oxygen species (ROS) on viral infection, while PC are likely linked homeostasis indicate toxicity host was affected infection. We propose prevents interfering DNA (deoxyribonucleic acid) synthesis through transcriptional block, which ultimately suppresses formation ROS, biochemical required for successful