Determination of L-carnitine in biological fluids and tissues.

摘要: In most biological materials, free L-carnitine is present together with short-chain and long-chain carnitine esters. These are differentiated mainly according to their solubility in aqueous solvents. A standardized extraction procedure therefore essential for reproducible estimations of content. Assays based on the reaction acetyl CoA formation CoASH, catalysed by transferase (EC 2.3.1.7). The two main principles employed monitor this a) measurement incorporation radio-labelled groups derived from into carnitine, b) photometric determination CoASH formed reaction, using thiol-group colour reagents or an enzymatic reaction. To avoid background due thiol-compounds sample, we suggest introduction oxidation step hydrogen peroxide, which then removed catalase. Using method, have established reference ranges total acid-soluble serum (men: 44.2-79.3, 34.8-69.5, women: 28.1-66.4 19.3-53.9 mumol/l, resp.), skeletal muscle (adults: 21.0-23.1, 19.5-35.1, children: 16.1-39.0 12.1-25.5 mumol/g non-collagen protein, urine. concentration acyl 2.0-4.0 mumol/l. Carnitine levels serum, tissues urine age-dependent lower newborn children. ratio a reflection hepatic production.(ABSTRACT TRUNCATED AT 250 WORDS)