Molecular and physical interactions of human sperm with female tract secretions

摘要: To achieve fertilisation, human sperm have to navigate and interact with the female reproductive tract (FRT) on molecular mechanical levels. The current knowledge of some aspects both types interactions are limited they were examined in this research. Proteomic analysis crude depleted follicular fluid (hFF) by three proteomic approaches identified 479 hFF-proteins which 22% novel. A table hFF-proteins, compiled from twenty-four hFF studies, resulted 1586 proteins; a resource for folliculogenesis discovery biomarkers. A comparative study media-capacitated versus capacitated presence revealed certain proteins acquired during capacitation. Comparative metabolomics elevated metabolites capacitation-media following 6-hour incubation compared 1-hour swim-up, may relevance energy metabolism potentially signalling mechanisms FRT causing remodelling fertilisation preparation implantation. Sperm micro-particle image-velocimetry an average velocity around motile ≤ 25- 45 μm/s 20-35 low high viscosity media respectively. averaged vorticity manifested trail spatially confined mixing surroundings sperm. best our knowledge, work is first conduct sperm-hFF interaction perform capacitation at two time points. novel perspective motility studying adds new approach chemotaxis. In conclusion, sperm-FRT involve complex physical regulatory events. Further research these enhance understanding potential applications improved assisted techniques’ outcome possible diagnostic infertility.