Conformation changes in rRNA induced by electric impulses

摘要: Abstract We have investigated the effects of electric field pulses (of about 44 kV/cm magnitude and 30 μsec duration) on E. coli ribosomal RNA in solution (5 × 10 −4 M sodium cacodylate buffer, pH 7, 20° C). The time course changes ultraviolet absorbance dichroism can be resolved into contributions from orientational conformational changes. fast part relaxation spectrum (completed less than 1 μs) is attributed to alignment double-helical segments rRNA with external field; slower (relaxation times ms μs range) shown due orientation entire τRNA molecule, concomitant field-induced helix-coil transitions oligomeric base-paired regions.