The proton-ATPase of bacteria and mitochondria.

摘要: We apologize for the fact that this review is longer than we intended; excuse ourselves with statement since almost all information have presented was discovered one of us last reviewed field in 1979, not strayed from topical. Remarkable advances understanding mechanism F1-catalyzed ATP hydrolysis and synthesis, structure proton-ATPase, genetics enzyme its assemblyin vivo been made four years. Understanding proton translocation across membrane seems now within our reach, but comprehension integration F1 catalysis remains somewhat elusive. feel roles ofuncF protein, OSCP F6 important regard. expect rapid chemical characterization mutation reversion sites by DNA sequencing will become possible near future, site-specific mutagenesis may soon allow to introduce amino acid substitutions specific domains subunits. Such capabilities would greatly expand horizons, allowing detailed molecular probing components proton-ATPase. are pleased had opportunity at an opportune time. Being both Senior wise shall be surprised or distressed if views interpretations fail elicit universal concurrence; do hope readers find them valuable stimulating.