Strain rate does not affect cortical microtubule orientation in the isolated epidermis of sunflower hypocotyls.

作者: A. Burian , Z. Hejnowicz

DOI: 10.1111/J.1438-8677.2009.00228.X

关键词: UltrastructureBiophysicsStrain rateMicrotubuleBiologyEpidermis (botany)Strain (chemistry)ElongationCell wallBotanyCortical microtubule

摘要: A hypothesis exists that external and internal factors affect the orientation of cortical microtubules in as much these lead to changes cell elongation rate. Factors stimulate are proposed transverse microtubule orientation, whereas inhibit longitudinal orientation. The rate is equal irreversible strain walls. Incubated epidermis peeled from sunflower hypocotyls does not extend unless it stretched by loading pH incubation medium appropriately low. Thus, peels provide a convenient model investigate relationship between In present study, was found peeling affects Peels were incubated for several hours Murashige & Skoog (both unbuffered buffered) attain steady state before loading. effects on under outer epidermal walls examined three portions positioned with respect cotyledonary node. Appropriate caused at 4.5 but 6.5. However, no clear effect observed. Independent applied load medium, remained unchanged, i.e. mainly oblique. Our results show isolated hypocotyl system, although could be changed white light.

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