Screen-printed biosensor modified with carbon black nanoparticles for the determination of paraoxon based on the inhibition of butyrylcholinesterase

摘要: We have developed a screen-printed electrochemical electrode (SPE) for paraoxon based on its inhibitory effect the enzyme butyrylcholinesterase (BChE). The was first modified by drop casting with dispersion of carbon black nanoparticles (CBNPs) in dimethylformamide-water mixture, and BChE then immobilized surface cross-linking. resulting biosensor exposed to standard solutions paraoxon, enzymatic hydrolysis butyrylthiocholine over time determined measuring product thiocholine at working voltage +300 mV. inhibition is linearly related concentration up 30 μg L−1, detection limit 5 μg L−1. stable 78 days storage room temperature under dry conditions. It applied spiked waste water samples. results underpin potential use CBNPs biosensors also demonstrate that they represent viable alternative other nanomaterials such as nanotubes or graphene, advantage being very affordable.