Optimizing methods in immunocytochemistry: One laboratory's experience

摘要: : The addition of immunocytochemical staining procedures to a diagnostic cytology service enables greater specificity interpretation for many common disease conditions, especially neoplastic diseases. However, well-tested immunohistochemical techniques may require modification cytologic specimens, and other considerations are necessary when working with air-dried cells. In this article, we describe our experience in evaluating options sample transport handling, discuss methods obtaining control cells from variety tissues use staining. Important principles techniques, including fixation, antigen retrieval, primary secondary antibodies manual automated systems described as used laboratory specimens. Although emphasize relevant laboratories receiving samples external clients, the information is also applicable any interested adding or enhancing services.