Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism
作者： Doris Albinsky , Miyako Kusano , Mieko Higuchi , Naomi Hayashi , Makoto Kobayashi
DOI: 10.1093/MP/SSP069
关键词: Biology 、 Metabolomics 、 Gene expression 、 Genetics 、 Botany 、 Gene 、 Metabolite 、 Plant cell 、 Arabidopsis 、 Complementary DNA 、 Transcriptome
摘要: Plant metabolomics developed as a powerful tool to examine gene functions and gain deeper insight into the physiology of plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length (FL) cDNAs (rice FOX lines) using gas chromatography-time-of-flight mass spectrometry (GC-TOF/MS)-based technique identify genes that caused metabolic changes. This screening system allows fast reliable identification candidate showing altered metabolite profiles. We performed metabolomic transcriptomic analysis line harbored FL cDNA ortholog Lateral Organ Boundaries (LOB) Domain (LBD)/Asymmetric Leaves2-like (ASL) Arabidopsis, At-LBD37/ASL39. The investigated showed prominent changes in levels metabolites related nitrogen metabolism. data well results from At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, Os-LBD37/ASL39-overexpressing indicated Os-LBD37/ASL39 is associated processes metabolism rice. Thus, combination metabolomics-based method gain-of-function approach useful for rapid characterization novel both
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