作者: Tao Wang , Lei Guo , Chong Lei , Yong Zhou
DOI: 10.1039/C5RA08642F
关键词: Quantitative determination 、 Sandwich immunoassay 、 Giant magnetoimpedance 、 Materials science 、 Antigen 、 Magnetic immunoassay 、 Dynabeads 、 Linear relationship 、 Detection limit 、 Nuclear magnetic resonance
摘要: Herein, a giant magnetoimpedance (GMI) sensor combined with the sandwich immunoassay was used for ultrasensitive detection of carcinoembryonic antigens (CEA). Oblong-shaped Au films were enclosed SU-8 photoresists to form several microcavities that treated different concentrations CEA. The quantification low-concentration CEA (1 pg mL−1–10 ng mL−1) achieved by using one single GMI sensor, demonstrating good linear relationship between response and concentration. lower limit found be as low 1 mL−1, which provides extreme sensitivity. We observed high-concentration (>10 can result in cluster Dynabeads, thereby reducing target signals since adjacent magnetic dipole fields canceled each other out. Target specificity GMI-based has also been verified testing nonspecific targets. results obtained here suggest could serve an immunosensor quantitative determination biomarkers.