Dual-enzyme cascade: an amplified method for the detection of alkaline phosphatase
作者： Patrick D. Mize , Randal A. Hoke , C.Preston Lin , John E. Reardon , Thomas H. Schulte
DOI: 10.1016/0003-2697(89)90120-6
关键词: Carboxylesterase 、 Alkaline phosphatase 、 Phosphate 、 Enzyme inhibitor 、 Enzyme 、 Biochemistry 、 Esterase 、 Acid phosphatase 、 Phosphatase 、 Chemistry
摘要: A method in which a two-enzyme cascade is used for rapid and sensitive detection of alkaline phosphatase described. masked inhibitor, 4-(3-oxo-4,4,4-trifluorobutyl)phenyl phosphate, dephosphorylated by the action phosphatase. The resulting compound, 1,1,1-trifluoro-4-(4-hydroxyphenyl)-butan-2-one, acts as potent inhibitor second enzyme, liver carboxylesterase. determination residual esterase activity provides highly indication original concentration. sensitivity this dual-enzyme approximately 125-fold greater than that observed direct with p-nitrophenyl phosphate.
elsevier.com
sci-hub.se 参考文章(14)
S.K. Miller, A.R. Main, R.S. Rush, Purification and physical properties of oligomeric and monomeric carboxylesterases from rabbit liver. Journal of Biological Chemistry. ,vol. 255, pp. 7161- 7167 ,(1980) , 10.1016/S0021-9258(20)79680-8
Sungman Cha, Tight-binding inhibitors—III Biochemical Pharmacology. ,vol. 25, pp. 2695- 2702 ,(1976) , 10.1016/0006-2952(76)90259-8
Bruce D. Hammock, Yehia A.I. Abdel-Aal, Christopher A. Mullin, Terry N. Hanzlik, Richard M. Roe, Substituted thiotrifluoropropanones as potent selective inhibitors of juvenile hormone esterase Pesticide Biochemistry and Physiology. ,vol. 22, pp. 209- 223 ,(1984) , 10.1016/0048-3575(84)90092-0
John F. Morrison, The slow-binding and slow, tight-binding inhibition of enzyme-catalysed reactions Trends in Biochemical Sciences. ,vol. 7, pp. 102- 105 ,(1982) , 10.1016/0968-0004(82)90157-8
Michael H. Gelb, John P. Svaren, Robert H. Abeles, Fluoro ketone inhibitors of hydrolytic enzymes. Biochemistry. ,vol. 24, pp. 1813- 1817 ,(1985) , 10.1021/BI00329A001
Michel Fosset, Danielle Chappelet-Tordo, Michel Lazdunski, Intestinal alkaline phosphatase. Physical properties and quaternary structure. Biochemistry. ,vol. 13, pp. 1783- 1788 ,(1974) , 10.1021/BI00706A001
Barbara Imperiali, Robert H. Abeles, inhibition of serine proteases by peptidyl fluoromethyl ketones Biochemistry. ,vol. 25, pp. 3760- 3767 ,(1986) , 10.1021/BI00361A005
Bruce D. Hammock, Keith D. Wing, James McLaughlin, Victor M. Lovell, Thomas C. Sparks, Trifluoromethylketones as possible transition state analog inhibitors of juvenile hormone esterase Pesticide Biochemistry and Physiology. ,vol. 17, pp. 76- 88 ,(1982) , 10.1016/0048-3575(82)90128-6
William P. Jencks, P. Greenzaid, Pig liver esterase. Reactions with alcohols, structure-reactivity correlations, and acyl-enzyme intermediate Biochemistry. ,vol. 10, pp. 1210- 1222 ,(1971) , 10.1021/BI00783A018
R Wolfenden, Transition State Analog Inhibitors and Enzyme Catalysis Annual Review of Biophysics and Bioengineering. ,vol. 5, pp. 271- 306 ,(1976) , 10.1146/ANNUREV.BB.05.060176.001415