Human 75-kDa DNA-pairing Protein Is Identical to the Pro-oncoprotein TLS/FUS and Is Able to Promote D-loop Formation
作者： David Ron , Bernard S. Lopez , Alexandre T. Akhmedov , Heidi Baechtold , Masahiko Kuroda
关键词: Replication protein A 、 Biology 、 DNA 、 Nuclear protein 、 Complementary DNA 、 Oligonucleotide 、 DNA repair 、 In vitro recombination 、 Molecular biology 、 Cell biology 、 Homologous recombination
摘要: Homologous recombination plays a fundamental role in DNA double-strand break repair. Previously, we detected two mammalian nuclear proteins of 100 and 75 kDa (POMp100 POMp75, respectively) that are able to promote homologous pairing, key step recombination. Here describe the identification human (h) POMp75 as pro-oncoprotein TLS/FUS. hPOMp75/TLS binds both single- double-stranded DNAs mediates annealing complementary strands. More important, it promotes uptake single-stranded oligonucleotide into superhelical form D-loop. The formation D-loop is an essential repair through catalyzed by require Mg2+ ATP-independent. Interestingly, oncogenic fusion TLS-CHOP not pairing. These data suggest possible for maintenance genomic integrity.
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