Caveolin-1 Confers Resistance of Hepatoma Cells to Anoikis by Activating IGF-1 Pathway.
作者： Wenqing Tang , Xuemei Feng , Si Zhang , Zhenggang Ren , Yinkun Liu
DOI: 10.1159/000430292
关键词: PI3K/AKT/mTOR pathway 、 Caveolin 1 、 Gene knockdown 、 Internalization 、 Cell biology 、 Protein kinase B 、 MAPK/ERK pathway 、 Caveolae 、 Anoikis 、 Biology
摘要: Background/Aims: Anoikis resistance is a prerequisite for hepatocellular carcinoma (HCC) metastasis. The role of Caveolin-1 (CAV1) in anoikis HCC remains unclear. Methods: oncogenic effect CAV1 on anchor-independent growth and was investigated by overexpression knockdown hepatoma cells. IGF-1 pathway its downstream signals were detected immunoblot analysis. Caveolae invagination IGF-1R internalization studied electron microscopy 125I-IGF1 assay, respectively. tyrosine-14 residue (Y-14) explored deletion experiment mutation experiment, correlation further examined immunochemical analysis 120 specimens. Results: could promote CAV1-overexpression increased the expression subsequently activated PI3K/Akt RAF/MEK/ERK pathway, while showed opposite effect. mechanism study revealed that facilitated caveolae internalization. or Y-14 reversed mediated resistance. In addition, positively related to human tissues. Conclusion: confers cells activating providing potential therapeutic target
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