Detection of TNT in Water Using an Evanescent Wave Fiber-Optic Biosensor
作者： Lisa C. Shriver-Lake , Kristen A. Breslin , Paul T. Charles , David W. Conrad , Joel P. Golden
DOI: 10.1021/AC00110A018
关键词: Matrix (chemical analysis) 、 Biosensor 、 Analyte 、 Fluorophore 、 Analytical chemistry 、 Chemistry 、 Trinitrotoluene 、 Buffer (optical fiber) 、 Contamination 、 Explosive material
摘要: A general health hazard from explosive toxicity exists due to poor storage facilities for explosives and contamination of soil water with byproducts manufacture. To provide an effective method monitoring contamination, the Naval Research Laboratory's fiber-optic biosensor was adapted detection small molecules such as explosives. competitive immunoassay developed most widely used explosive, trinitrotoluene (TNT). Trinitrobenzenesulfonic acid (TNB), analog TNT, labeled a fluorophore analyte competitor. solution containing 7.5 ng/mL sulfoindocyanine 5-ethyl-enediamine-labeled TNB (Cy5-EDA-TNB) exposed antibody-coated optical fiber, generating specific signal above background that corresponds 100% or reference signal. Inhibition proportional TNT concentration in sample. Detection sensitivities 10 (8 ppb) buffer were achieved. Tests on river, harbor, bilge samples spiked showed results similar tests laboratory (buffer matrix) samples. determine possible interferences, several structural analogs ofTNT tested.
acs.org
sci-hub.se 参考文章(7)
Dean D. Fetterolf, James L. Mudd, Karen Teten, An enzyme-linked immunosorbent assay (ELISA) for trinitrotoluene (TNT) residue on hands. Journal of Forensic Sciences. ,vol. 36, pp. 343- 349 ,(1991) , 10.1520/JFS13036J
Yunke Zhang, W.Rudolf Seitz, C.L. Grant, Donald C. Sundberg, A clear, amine-containing poly(vinyl chloride) membrane for in situ optical detection of 2,4,6-trinitrotoluene Analytica Chimica Acta. ,vol. 217, pp. 217- 227 ,(1989) , 10.1016/S0003-2670(00)80404-3
Suresh K. Bhatia, Lisa C. Shriver-Lake, Kimberly J. Prior, Jacque H. Georger, Jeffrey M. Calvert, Reinhard Bredehorst, Frances S. Ligler, Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces. Analytical Biochemistry. ,vol. 178, pp. 408- 413 ,(1989) , 10.1016/0003-2697(89)90662-3
Yunke Zhang, W.Rudolf Seitz, Single fiber absorption measurements for remote detection of 2,4,6-trinitrotoluene Analytica Chimica Acta. ,vol. 221, pp. 1- 9 ,(1989) , 10.1016/S0003-2670(00)81933-9
Joel P Golden, Lisa C Shriver-Lake, George P Anderson, Richard B Thompson, Frances S Ligler, Fluorometer and tapered fiber optic probes for sensing in the evanscent wave Optical Engineering. ,vol. 31, pp. 1458- 1462 ,(1992) , 10.1117/12.58764
G.P. Anderson, J.P. Golden, L.K. Cao, D. Wijesuriya, L.C. Shriver-Lake, F.S. Ligler, Development of an evanescent wave fiber optic biosensor IEEE Engineering in Medicine and Biology Magazine. ,vol. 13, pp. 358- 363 ,(1994) , 10.1109/51.294007
Thomas F. Jenkins, Paul H. Miyares, Karen F. Myers, Erika F. McCormick, Ann B. Strong, Comparison of solid phase extraction with salting-out solvent extraction for preconcentration of nitroaromatic and nitramine explosives from water Analytica Chimica Acta. ,vol. 289, pp. 69- 78 ,(1994) , 10.1016/0003-2670(94)80009-X