Method of producing recombinant adamts13 in cell culture
作者： Leopold Grillberger , Manfred Reiter , Daniel Fleischanderl , Gregor Bramberger
DOI:
关键词: Specific activity 、 Cell culture 、 ADAMTS13 、 Biochemistry 、 Biology 、 Molecular biology 、 Increased copper 、 Recombinant DNA 、 Cell culture supernatant 、 Ammonium
摘要: Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a specific activity and ADAMTS13 activity. The of can include, example, medium an increased copper concentration and/or supernatant low ammonium (NH4 +) concentration. also provides methods cultivating cells express vWF rA13 having activities.
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