Chitin deacetylase family genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae).

摘要: Chitin deacetylases (CDAs) are enzymes required for one of the pathways chitin degradation, in which chitosan is produced by deacetylation chitin. Bioinformatic investigations with genomic and transcriptomic databases identified four genes encoding CDAs Nilaparvata lugens (NlCDAs). Phylogenetic analysis showed that insect were clustered into five major groups. Group I, III IV found all species, whereas pupa-specific group II gut-specific V not plant-sap/blood-sucking hemimetabolous species from Hemiptera Anoplura. The developmental tissue-specific expression patterns NlCDAs revealed NlCDA3 was a CDA, high at stages; NlCDA1, NlCDA2 NlCDA4 highly expressed integument peaked periodically during every moulting, suggests their roles turnover old cuticle. Lethal phenotypes cuticle shedding failure mortality after injection double-stranded RNAs (dsRNAs) provide further evidence functions associated moulting. No observable morphological internal structural abnormality obtained insects treated dsRNA NlCDA3.