Lactate Measurements with an Enzyme Optode that Uses Two Oxygen Fluorescence Indicators to Measure the PO2 Gradient Directly

摘要: The main topic of this symposium is the measurement ions such as K+, Na+, Ca2+ etc. by means potential differences produced specific ion carriers across a fluid layer or suitable membrane. I would like to mention that absorption well fluorescence measurements with indicators represent additional approaches these ions. difficulty often react other substances in sample whereby calibration curve changed. This restricts their applicability biology. Our experiments have shown can be overcome introducing specialized membrane between probe and which allows only passage those molecules do not disturb optical signals. compartmentation has several advantages: 1) indicator dissolved liquids could sample, 2) used for an separation from 3) some cases directly bound Fluorescence are especially effective (3f 4), because high sensitivity.