Tumor necrosis factor-alpha gene and protein expression in adult feline myocardium after endotoxin administration.

摘要: TNF alpha mRNA and protein biosynthesis were examined in the adult feline heart after stimulation with endotoxin. When freshly isolated hearts stimulated endotoxin vitro, de novo expression occurred within 30 min, production was detected 60-75 min; however, not diluent-treated hearts. Immunohistochemical studies localized to endothelial cells, smooth muscle cardiac myocytes endotoxin-treated hearts, whereas immunostaining absent To determine whether myocyte a source for production, two performed. First, situ hybridization studies, using highly specific biotinylated probes, demonstrated from endotoxin-stimulated hearts; contrast, expressed Second, observed when cultured endotoxin, cells. The functional significance of intramyocardial determined by examining cell motion treated superfusates endotoxin- diluent-stimulated These showed that depressed but normal moreover, negative inotropic effects could be abrogated completely pretreatment an anti-TNF antibody. Finally, also shown result vivo. Thus, this study shows first time mammalian myocardium synthesizes biologically active alpha.