Confocal Raman spectroscopic imaging for in vitro monitoring of active ingredient penetration and distribution in reconstructed human epidermis model.

摘要: Topically applied active cosmetic ingredients (ACI) or pharmaceutical (API) efficacy is directly related to their efficiency of penetration in the skin. In vitro reconstructed human epidermis surrogate models offer vivo like skin samples for transdermal studies. Using Delipidol®, an ACI currently used cosmetics industry, capabilities deliver accurate distribution maps and profiles this molecule by means confocal Raman spectroscopic imaging have been demonstrated. a non-negative constrained least squares (NCLS) approach, contribution specific molecules can be estimated at each point spectral order semi-quantitative heat representing levels different layers. The concentration obtained are approximately single exponential all 3 time points evaluated, with consistent decay constant, which independent sublayer structure. Notably, however, there no significant into lower basal layers until critical built up, after hours. Combination unmixing methods such as NCLS demonstrated relevant approach biological evaluation could easily implemented screening tool industrial use.