Structure of the archaeal transfer RNA nucleoside G*-15 (2-amino-4,7-dihydro- 4-oxo-7-beta-D-ribofuranosyl-1H-pyrrolo[2,3-d]pyrimidine-5-carboximi dam ide (archaeosine)).

摘要: A number of post-transcriptional modifications in tRNA are phylogenetically characteristic the bacterial, eukaryal, or archaeal domains, both with respect to sequence location and molecular structure at nucleoside level. One most distinct such is G*, located position 15, which bacterial eukaryal tRNAs a conserved site involved maintenance dihydrouridine loop-T-loop tertiary interactions. G* occurs widely nearly every branch phylogenetic domain, contrast its absence all reported sequences. The G*-15 2-amino-4,7-dihydro-4-oxo-7-beta-D-ribofuranosyl-1H- pyrrolo[2,3-d]pyrimidine-5-carboximidamide (7-formamidino-7-deazaguanosine), non-purine, non-pyrimidine ribonucleoside; thus reflects extensive modification beyond guanine-15 specified by corresponding gene was established mass spectrometry, particular from collision-induced dissociation spectra derivatives formed microscale permethylation, confirmed chemical synthesis.