Vasoactive intestinal peptide and helodermin inhibit phospholipase A2 activity in vitro
作者： Margit E. Trotz , Sami I. Said
DOI: 10.1016/0167-0115(93)90158-5
关键词: Peptide 、 Secretin 、 Biology 、 Phospholipase A 、 Vasoactive intestinal peptide 、 Biochemistry 、 Arachidonic acid 、 Lipoxygenase 、 Phospholipase A2 、 Lung injury
摘要: Abstract We recently reported that the widely distributed neuropeptide vasoactive intestinal peptide (VIP) reduces inflammatory lung injury due to a variety of agents and inhibits associated generation cyclo-oxygenase lipoxygenase products. therefore investigated whether VIP may inhibit phospholipase A 2 activity, thus reducing release arachidonic acid, common precursor all eicosanoids. dose-dependently inhibited PLA porcine pancreas Naja naja venom, as assessed by free [ 3 H]oleic acid from labeled Escherichia coli phospholipids. The potency was similar mepacrine, with 50% inhibition at 400–500 μM. closely related helodermin produced 200 μM, but secretin histidine isoleucineamide little or no inhibition. results suggest selectively in vitro. If this activity is exerted vivo, it contribute anti-inflammatory these two peptides.
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