De novo transcriptome analysis of Cnidium monnieri (L.) Cuss and detection of genes related to coumarin biosynthesis

摘要: Cnidium monnieri (L.) Cuss (C. monnieri) is one of the most widely used traditional herbal medicines, exhibiting a wide range pharmacological functions for treating asynodia, trichomonas vaginitis, and osphyalgia. Its important medicinal value comes from its abundance coumarins. To identify genes involved in coumarin biosynthesis accumulation, we analyzed transcriptome data flower, leaf, root stem tissues C. monnieri. A total 173,938 unigenes with mean length 1,272 bp, GC content 38.79%, N50 2,121 bp were assembled using Trinity program. Of these, 119,177 annotated public databases. We identified differentially expressed (DEGs) based on expression profile analysis. These DEGs exhibited higher levels flower tissue than or tissues. numerous encoding enzymes biosynthesis, verified key quantitative real-time PCR. Our will make great contributions to research provide clues identifying candidate metabolic pathways.