Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae
作者： Minrui Guo , Guogang Chen , Jiluan Chen , Minggang Zheng
DOI: 10.1007/S11802-019-3946-Y
关键词: Biochemistry 、 Amino acid 、 Heterologous expression 、 Fatty acid 、 Biosynthesis 、 Saccharomyces cerevisiae 、 Linolenic acid 、 Long chain fatty acid 、 Chemistry 、 Polyunsaturated fatty acid 、 Ocean Engineering 、 Oceanography
摘要: The marine microalga Nannochloropsis sp. contains various elongases and desaturases that are critical for biosynthesis of polyunsaturated fatty acids. A full-length cDNA encoding a long-chain acid elongase, named NsFAE, was cloned from sp.. open reading frame NsFAE (GenBank accession no. MF680548) consisted 1068bp encoded predicted protein 355 amino acids with molecular mass 38.8kDa. deduced polypeptide showed 43%-44% identity to acyl other algae. RT-PCR experiments indicated the gene exhibited highest expression in at 72h (i.e., during third growth stage) significantly lower four stages. Plasmid pNsFAE-CRISPR recombinant DNA fragment (ADH1p-NsFAE-CYCt) were transformed into Saccharomyces cerevisiae strain BY4742 using CRISPR-Cas system. Yeast transformants containing produced three not normally present wild-type BY4742-linoleic acid, linolenic eicosadienoic acid-indicating encodes functional elongase enzyme.
harvard.edu
springer.com
sci-hub.se 参考文章(39)
James R. Petrie, Anne M. Mackenzie, Pushkar Shrestha, Qing Liu, Dion F. Frampton, Stan S. Robert, Surinder P. Singh, Isolation of three novel long-chain polyunsaturated fatty acid Δ9-elongases and the transgenic assembly of the entire Pavlova salina docosahexaenoic acid pathway in Nicotiana benthamiana. Journal of Phycology. ,vol. 46, pp. 917- 925 ,(2010) , 10.1111/J.1529-8817.2010.00870.X
Jun Xia, Ling Wang, Jian-bo Zhu, Cheng-jun Sun, Ming-gang Zheng, Li Zheng, Ying-hua Lou, Lei Shi, Expression of Shewanella frigidimarina fatty acid metabolic genes in E. coli by CRISPR/cas9-coupled lambda Red recombineering Biotechnology Letters. ,vol. 38, pp. 117- 122 ,(2016) , 10.1007/S10529-015-1956-4
Songsri Kaewsuwan, Nantavan Bunyapraphatsara, David J. Cove, Ralph S. Quatrano, Pichit Chodok, High level production of adrenic acid in Physcomitrella patens using the algae Pavlova sp. Δ5-elongase gene Bioresource Technology. ,vol. 101, pp. 4081- 4088 ,(2010) , 10.1016/J.BIORTECH.2009.12.138
R Montes, A M Chisaguano, A I Castellote, E Morales, J Sunyer, M C López-Sabater, Fatty-acid composition of maternal and umbilical cord plasma and early childhood atopic eczema in a Spanish cohort. European Journal of Clinical Nutrition. ,vol. 67, pp. 658- 663 ,(2013) , 10.1038/EJCN.2013.68
Elisa Corteggiani Carpinelli, Andrea Telatin, Nicola Vitulo, Claudio Forcato, Michela D’Angelo, Riccardo Schiavon, Alessandro Vezzi, Giorgio Mario Giacometti, Tomas Morosinotto, Giorgio Valle, Chromosome scale genome assembly and transcriptome profiling of Nannochloropsis gaditana in nitrogen depletion. Molecular Plant. ,vol. 7, pp. 323- 335 ,(2014) , 10.1093/MP/SST120
Baoxiu Qi, Frédéric Beaudoin, Tom Fraser, A.Keith Stobart, Johnathan A Napier, Colin M Lazarus, Identification of a cDNA encoding a novel C18-Δ9 polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana1 FEBS Letters. ,vol. 510, pp. 159- 165 ,(2002) , 10.1016/S0014-5793(01)03247-1
Pichit Chodok, Pradinunt Eiamsa-ard, David J. Cove, Ralph S. Quatrano, Sireewan Kaewsuwan, Identification and functional characterization of two Δ12-fatty acid desaturases associated with essential linoleic acid biosynthesis in Physcomitrella patens Journal of Industrial Microbiology & Biotechnology. ,vol. 40, pp. 901- 913 ,(2013) , 10.1007/S10295-013-1285-3
Christopher Leber, Brian Polson, Ruben Fernandez-Moya, Nancy A. Da Silva, Overproduction and secretion of free fatty acids through disrupted neutral lipid recycle in Saccharomyces cerevisiae. Metabolic Engineering. ,vol. 28, pp. 54- 62 ,(2015) , 10.1016/J.YMBEN.2014.11.006
Toshio Takeuchi, A review of feed development for early life stages of marine finfish in Japan Aquaculture. ,vol. 200, pp. 203- 222 ,(2001) , 10.1016/S0044-8486(01)00701-3
Stan S. Robert, Surinder P. Singh, Xue-Rong Zhou, James R. Petrie, Susan I. Blackburn, Peter M. Mansour, Peter D. Nichols, Qing Liu, Allan G. Green, Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed oil Functional Plant Biology. ,vol. 32, pp. 473- 479 ,(2005) , 10.1071/FP05084