Polymer therapy: a novel treatment to improve fat graft viability.

摘要: Background: Autologous fat grafting is currently undergoing a renaissance. However, grafts are limited by unpredictable survival. Poloxamers can act as tissue surfactants. These nonionic surfactants have been shown to stabilize the membranes of damaged cells and protect against injury apoptosis in numerous models. This study was designed investigate ability poloxamers harvested adipocytes increase graft Methods: Lipoaspirate obtained from surgical patients. Samples were washed normal saline, centrifuged at 200 g, treated with various or poloxamer components for 30 minutes, implanted into flanks nude mice 1.0-cc, 1.0-g lobules. The explanted serially 10 days 6 weeks. Endpoints weight, apoptosis, cell viability, DNA content, histology. Results: Grafts P188, F108, F127 demonstrated increased survival weight. Fat L64 P188 improvement those L64, F38 improved P188-treated 50 percent reduction compared saline-treated controls (p < 0.05) an overall 72 weight statistically significant histology, viability (89 versus 33 percent). Conclusions: authors demonstrate that poloxamers, membrane-sealing capability, Among these