Molecular analysis of the biomass of a fluidized bed reactor treating synthetic vinasse at anaerobic and micro-aerobic conditions

摘要: The microbial communities (Bacteria and Archaea) established in an anaerobic fluidized bed reactor used to treat synthetic vinasse (betaine, glucose, acetate, propionate, butyrate) were characterized by denaturing gradient gel electrophoresis (DGGE) phylogenetic analysis. This study was focused on the competitive syntrophic interactions between different groups at varying influent substrate sulfate ratios of 8, 4, 2 or micro-aerobic conditions. Acetogens detected along phases 8 4 seemed be mainly involved fermentation glucose betaine, but they substituted other sugar betaine degraders after oxygen application. Typical fatty acid that grow syntrophy with methanogens not during entire run. Likely, outnumbered them DGGE sulfate-reducing bacteria (SRB) belonged hydrogen-utilizing Desulfovibrio. introduction led formation elemental sulfur (S0) probably compounds sulfide-oxidizing (γ-Proteobacteria). It is likely intermediates produced from sulfide oxidation SRB microorganisms as electron acceptors, supported detection respiring Wolinella succinogenes. Within Archaea population, members Methanomethylovorans Methanosaeta throughout operation. Hydrogenotrophic belonging genus Methanobacterium highest ratio rapidly disappeared increasing concentration.