QUANTITATIVE ANALYSIS OF DIACETYL, PENTANEDIONE AND THEIR PRECURSORS DURING BEER FERMENTATION BY AN ACCURATE GC/MS METHOD
作者： Sophie Landaud , Pascale Lieben , Daniel Picque
DOI: 10.1002/J.2050-0416.1998.TB00981.X
关键词: Gas chromatography 、 Toluene 、 Vicinal 、 Gas chromatography–mass spectrometry 、 Detection limit 、 Diketone 、 Derivatization 、 Diacetyl 、 Chromatography 、 Chemistry
摘要: A GC/MS method previously described for diacetyl was developed the quantification of 2,3-pentanedione, and derivatization procedure modified determination α-acetohydroxy acid. The reaction 2,3-pentanedione with 4,5-dichloro-1,2-diaminobenzene produced 6,7-dichloro-2-methyl-3-ethylquinoxaline (DCMEQ), which extracted toluene determined by gas chromatography using a mass selective detector. formation DCMEQ linearly correlated concentration. very simple sensitive. detection limit derivative 0.0007 mg/litre 0.0002 extract respectively, 0.001 mg/litre, respectively. Cautious sample treatment led to low (10%) controlled conversion acids vicinal diketones. This reproducible percentage made it possible determine precisely free diketones acids. The applied precursors concentrations during beer fermentation.
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