A novel MDMX transcript expressed in a variety of transformed cell lines encodes a truncated protein with potent p53 repressive activity.

摘要: The MDMX gene product is related to the MDM2 oncoprotein, both of which interact with p53 tumor suppressor. We have identified a novel transcript that expressed in variety cell lines, and particular, growing transformed cells. This identical published sequence yet it has short internal deletion 68 base pairs. produces shift reading frame after codon 114, resulting inclusion stop at amino acid residue 127 (full-length 489 residues). truncated protein termed MDMX-S ("short form"), represents only p53-binding domain, appears bind better than full-length MDMX. can be detected extracts when overexpressed much more effective inhibiting p53-mediated transcriptional activation induction apoptosis. Since lacks central carboxyl-terminal regions contained within MDMX, likely play key role regulation proliferation apoptosis way distinct from