Use of Engineered Nanoparticle-Based Fluorescence Methods for Live-Cell Phenomena
作者： Chan-Gi Pack , Min-Kyo Jung , Mi-Roung Song , Jun-Sung Kim , Sung-Sik Han
DOI: 10.1016/B978-0-12-409513-7.00011-7
关键词: Chemistry 、 Engineered Nanoparticle 、 Autophagosome 、 Nanoparticle 、 Vesicle 、 Cell biology 、 Fluorescence correlation spectroscopy 、 Endocytosis 、 Electroporation 、 Green fluorescent protein
摘要: For understanding the biophysical state of nanomolecules and nanoparticles (NPs) inside a live cell, cellular viscosity represented by diffusion is evaluated with tandem enhanced green fluorescent protein (EGFP) multimers as standard. Inert silica-based (Si-FNPs) combined electroporation are then used to evaluate free single Si-FNPs in cytosolic microenvironment. The uptake into cell quantitatively compared diffusion, results have demonstrated that NPs can freely diffuse cells coefficients determined hydrodynamic size viscosity. Three types mobility during process endocytosis been identified for FNPs. Interestingly, it was found strongly colocalized GFP–LC3, an autophagosome marker protein. approach based on interaction will provide insights developing strategies further development intercellular event-oriented well delivery living cells.
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