Low-dose gold nanoparticles exert subtle endocrine-modulating effects on the ovarian steroidogenic pathway ex vivo independent of oxidative stress
作者： Jeremy K. Larson , Michael J. Carvan , Justin G. Teeguarden , Gen Watanabe , Kazuyoshi Taya
DOI: 10.3109/17435390.2013.837208
关键词: Oxidative stress 、 HSD3B1 、 Endocrine system 、 Biology 、 Ex vivo 、 Nanotoxicology 、 Cholesterol side-chain cleavage enzyme 、 Hormone 、 CYP17A1 、 Internal medicine 、 Endocrinology
摘要: Gold nanoparticles (GNPs) have gained considerable attention for application in science and industry. However, the untoward effects of such particles on female fertility remain unclear. The objectives this study were to (1) examine 10-nm GNPs progesterone estradiol-17β accumulation by rat ovaries ex vivo (2) identify locus/loci whereby modulate steroidogenesis via multiple-reference gene quantitative real-time RT-PCR. Regression analyses indicated a positive relationship between both Star (p < 0.05, r(2) = 0.278) Cyp11a1 0.001, 0.366) expression P4 upon exposure 1.43 × 10(6) GNPs/mL. Additional showed that E2 was positively associated with Hsd3b1 0.181) Cyp17a1 0.01, 0.301) 1(3) 10(9) GNPs/mL, respectively. These results suggest subtle treatment-dependent impact low-dose or specific steroidogenic target genes, independent oxidative stress inhibin.
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