A unique C2 domain at the C terminus of Munc13 promotes synaptic vesicle priming
作者： Jeremy S. Dittman , Murugesh Padmanarayana , R. Bryan Sutton , Zhitao Hu , Matthew J. Dominguez
关键词: Protein family 、 C2 domain 、 Synaptic vesicle priming 、 Cell biology 、 Neurotransmitter 、 Chemistry 、 Protein domain 、 Vesicle 、 Synaptic vesicle 、 Neurotransmission
摘要: Neurotransmitter release during synaptic transmission comprises a tightly orchestrated sequence of molecular events, and Munc13-1 is cornerstone the fusion machinery. A forward genetic screen for defects in neurotransmitter Caenorhabditis elegans identified mutation ortholog UNC-13 that eliminated its unique deeply conserved C-terminal module (referred to as HC2M) containing Ca2+-insensitive C2 domain flanked by membrane-binding helices. The HC2M could be functionally replaced vivo protein domains localize vesicles but not plasma membrane. broadly other Unc13 family members required efficient vesicle priming. We propose evolved vesicle/endosome adaptor acquired specificity Unc13ABC family.
uq.edu.au参考文章(72)
Stephen H. White, William C. Wimley, Alexey S. Ladokhin, Kalina Hristova, Protein Folding in Membranes: Determining Energetics of Peptide-Bilayer Interactions. Methods in Enzymology. ,vol. 295, pp. 62- 87 ,(1998) , 10.1016/S0076-6879(98)95035-2
Xiaoyu Yang, Shen Wang, Yi Sheng, Mingshu Zhang, Wenjuan Zou, Lijie Wu, Lijun Kang, Josep Rizo, Rongguang Zhang, Tao Xu, Cong Ma, Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming Nature Structural & Molecular Biology. ,vol. 22, pp. 547- 554 ,(2015) , 10.1038/NSMB.3038
Bharathi Aravamudan, Tim Fergestad, Warren S Davis, Chris K Rodesch, Kendal Broadie, None, Drosophila Unc-13 is essential for synaptic transmission Nature Neuroscience. ,vol. 2, pp. 965- 971 ,(1999) , 10.1038/14764
Janet E. Richmond, Robby M. Weimer, Erik M. Jorgensen, An open form of syntaxin bypasses the requirement for UNC-13 in vesicle priming Nature. ,vol. 412, pp. 338- 341 ,(2001) , 10.1038/35085583
Janet E. Richmond, Erik M. Jorgensen, One GABA and two acetylcholine receptors function at the C. elegans neuromuscular junction Nature Neuroscience. ,vol. 2, pp. 791- 797 ,(1999) , 10.1038/12160
Iris Augustin, Christian Rosenmund, Thomas C. Südhof, Nils Brose, Munc13-1 is essential for fusion competence of glutamatergic synaptic vesicles Nature. ,vol. 400, pp. 457- 461 ,(1999) , 10.1038/22768
Janet E. Richmond, Warren S. Davis, Erik M. Jorgensen, UNC-13 is required for synaptic vesicle fusion in C. elegans Nature Neuroscience. ,vol. 2, pp. 959- 964 ,(1999) , 10.1038/14755
Stephen R. Proulx, Karen J. Yook, Erik M. Jorgensen, Rules of Nonallelic Noncomplementation at the Synapse in Caenorhabditis elegans Genetics. ,vol. 158, pp. 209- 220 ,(2001) , 10.1093/GENETICS/158.1.209
S Brenner, THE GENETICS OF CAENORHABDITIS ELEGANS Genetics. ,vol. 77, pp. 71- 94 ,(1974) , 10.1093/GENETICS/77.1.71
J. S. Dittman, J. M. Kaplan, Behavioral Impact of Neurotransmitter-Activated G-Protein-Coupled Receptors : Muscarinic and GABAB Receptors Regulate Caenorhabditis elegans Locomotion The Journal of Neuroscience. ,vol. 28, pp. 7104- 7112 ,(2008) , 10.1523/JNEUROSCI.0378-08.2008