Simultaneous quantification of cell motility and protein-membrane-association using active contours.

摘要: We present a new method for the quantification of dynamic changes in fluorescence intensities at cell membrane moving cells. It is based on an active contour cell-edge detection, which allows tracking shape and position. Fluorescence specific cortical subregions can be followed space time correlated with motility. The translocation two GFP tagged proteins (CRAC GRP1) from cytosol to response stimulation chemoattractant cAMP during chemotaxis Dictyostelium cells studies spatio-temporal dynamics this process exemplify method: show that motility parameters quantitative differences rate association dissociation observed PH domain containing proteins. analysis periodic CRAC leading edge responding natural waves mound demonstrates power approach. not only capable outline within aggregates front noisy background, but furthermore construction polar plots, capturing protein distribution cells' co-ordinate system. Compilation data by means normalised plots suggested as future tool, promises so-far impossible practicability extensive statistical automated comparison complex patterns.